ELISA Blocking Buffer - Non-Mammalian-based

• Description:

  This buffer is Non-Mammalian-based in origin and is designed primarily for antigen-down ELISA formats as well as sandwich assays with high background issues. It is particularly useful with mammalian serum samples, reducing sample-blocker interactions.

• CAS Number:

  9000-83-3

• Assay Principle:

  1. Prepare samples and controls, 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20., 3. Reconstitute FLICA with 50 µL DMSO., 4. Dilute FLICA 1:5 by adding 200 µL PBS., 5. Add diluted FLICA to each sample at 1:30 (e.g., add 10 µL to 290 µL of cultured cells)., 6. Incubate approximately 1 hour., 7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells., 8. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody., 9. If desired, fix cells., 10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLICA excites at 492 nm and emits at 520 nm., , If working with adherent cells, please see the manual for additional protocols.

• Concentration:

  1X

• pH:

  pH: 7.4 at 1X

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  4°C

• Shelf Life:

  24 months from date of manufacture