ELISA Blocking Buffer - Non-Mammalian-based

CAT: 0246-64Size: 1 LDry Ice: NoHazardous: No
CAT#:0246-64Size:1 L
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Description
This buffer is Non-Mammalian-based in origin and is designed primarily for antigen-down ELISA formats as well as sandwich assays with high background issues. It is particularly useful with mammalian serum samples, reducing sample-blocker interactions.
CAS Number
9000-83-3
Assay Principle
1. Prepare samples and controls, 2. Dilute 10X Apoptosis Wash Buffer 1:10 with diH20., 3. Reconstitute FLICA with 50 µL DMSO., 4. Dilute FLICA 1:5 by adding 200 µL PBS., 5. Add diluted FLICA to each sample at 1:30 (e.g., add 10 µL to 290 µL of cultured cells)., 6. Incubate approximately 1 hour., 7. Remove media and wash cells 3 times: add 1X Apoptosis Wash Buffer and spin cells., 8. If desired, label with additional stains, such as Hoechst, Propidium Iodide, 7-AAD, or an antibody., 9. If desired, fix cells., 10. Analyze with a fluorescence microscope, fluorescence plate reader, or flow cytometer. FAM-FLICA excites at 492 nm and emits at 520 nm., , If working with adherent cells, please see the manual for additional protocols.
Concentration
1X
pH
pH: 7.4 at 1X
Shipping Conditions
Blue Ice
Storage Conditions
4°C
Shelf Life
24 months from date of manufacture

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