ELISA Blocking Buffer

CAT:

246-643

Size:

1 L

Price:

Ask

For price, please contact [email protected]

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

ELISA Blocking Buffer

Description: Eliminate interference and nonspecific background associated with antibody-coated ELISA formats and sandwich ELISAs. ELISA Blocking Buffer (formerly known as Synblock) provides low background performance without the use of conventional cross-reactive protein additives.
CAS Number: 9000-83-3
Assay Principle: 1. Dilute 5X Antibody Coating Buffer 1:5 with deionized water and mix for 15 minutes. As Antibody Coating Buffer is a 5X concentrate, crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm the buffer until all crystals are dissolved. Do not let it boil., 2. Dilute your antibody into the coating buffer. Optimal coating concentration varies significantly from 0.1 µg/mL to 10 µg/mL., 3. Let the solution stir 10-15 minutes and pipette onto the plate. Optimal coating volume generally ranges between 50-200 µL per well. ICT recommends coating antibodies onto Costar 96-Well EIA/RIA Stripwell plate., 4. Once added to the plate, incubate the coating solution from 8-24 hours at room temperature protected from light. Minimize evaporation by individually covering each plate with a plate sealing cover, wrapping a stack in plastic wrap, or placing plates in a humidified storage box and covering., 5. Aspirate the coating solution., 6. Wash each well twice with 1X ELISA Wash Buffer (catalog #652)., 7. Block the uncoated regions of the microplate wells by pipetting 300 μL of blocking buffer (such as catalog #640, 64, or 643) into each well., 8. Incubate 8-24 hours at room temperature., 9. Aspirate the blocking buffer., 10. The assay can be run at this point, or the plate can be dried and packaged for later use., 11. Dry the plate by letting it sit on the bench top from 8-24 hours while protected from light, or dry in a drying chamber under vacuum from 3 – 6 hours at room temperature. When dry, seal the plate in an air-tight foil pouch with a desiccant packet and store at RT or 2-8°C protected from light.
Concentration: 1X
pH: pH: 7.4 at 1X
Shipping Conditions: Blue Ice
Storage Conditions: 4°C
Shelf Life: 24 months from date of manufacture

Related Products

Cat	Name
642	ELISA Blocking Buffer
641	ELISA Blocking Buffer
643	ELISA Blocking Buffer
6296	Monster Block ELISA Blocking Buffer
6299	Alternative Block ELISA Blocking Buffer
6300	Alternative Block ELISA Blocking Buffer
640	General Block ELISA Blocking Buffer
6295	Monster Block ELISA Blocking Buffer
6297	Monster Block ELISA Blocking Buffer
6301	Alternative Block ELISA Blocking Buffer

Alternative Products

Cat	Name
E104	ELISA Blocking Buffer
STEM34	ELISA Blocking Buffer
ML044-100ML	Blocking Buffer
abx098972-02	Blocking Buffer
E-ELIR-003-01	ELISA Plate Blocking Buffer
E-ELIR-003-02	ELISA Plate Blocking Buffer
MBS2571969-01	ELISA Plate Blocking Buffer
MBS258400-04	SynBlock ELISA Blocking Buffer
MBS318404-02	Blocking Buffer for ELISA
GWB-E120C6	Blocking Buffer for ELISA