DOTAP (methylsulfate)

• Description:

  DOTAP methylsulfat is a cationic lipid reagent, a cationic derivative of trimethylammonium, linked to two 18-carbon fatty acid tails, each with a single olefin group. DOTAP methylsulfat can self-assemble with negatively charged ions (such as DNA) to form complexes, which can be adsorbed to the cell membrane surface and enter the cell by electrostatic interaction and endocytosis, respectively. DOTAP methylsulfat promotes endosomal membrane fusion with its own hydrophobic domain, releases DNA into the cytoplasm, and exerts gene delivery function. DOTAP methylsulfat can be widely used in research fields such as gene therapy, cell transfection, and non-viral vector design[1][2].

• Product Name Alternative:

  1,2-Dioleoyl-3-trimethylammonium-propane (methylsulfate)

• UNSPSC:

  12352200

• Hazard Statement:

  H302, H315, H319, H331, H336, H351, H361, H372

• Target:

  Liposome

• Type:

  Biochemical Assay Reagents

• Related Pathways:

  Metabolic Enzyme/Protease

• Field of Research:

  Others

• Assay Protocol:

  https://www.medchemexpress.com/dotap-methylsulfate.html

• Purity:

  99.94

• Solubility:

  DMSO : 5 mg/mL (ultrasonic) |Ethanol : 100 mg/mL

• Smiles:

  S(OC)(=O)(=O)[O-].C(OC(CCCCCCC/C=C\CCCCCCCC)=O)(COC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C

• Molecular Formula:

  C43H83NO8S

• Molecular Weight:

  774.19

• Precautions:

  H302, H315, H319, H331, H336, H351, H361, H372

• References & Citations:

  [1]Kim BK, et al. DOTAP/DOPE ratio and cell type determine transfection efficiency with DOTAP-liposomes. Biochim Biophys Acta. 2015 Oct;1848 (10 Pt A) :1996-2001.|[2]Crook K, et al. Inclusion of cholesterol in DOTAP transfection complexes increases the delivery of DNA to cells in vitro in the presence of serum. Gene Ther. 1998 Jan;5 (1) :137-43.

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  4°C (Powder, sealed storage, away from moisture)

• Scientific Category:

  Biochemical Assay Reagents

• Clinical Information:

  Phase 2

• Citation 01:

  Signal Transduct Target Ther. 2025 Mar 24;10 (1) :101.|Adv Healthc Mater. 2023 Jan;12 (6) :e2202412.|bioRxiv. 2025 Oct 1.|Exploration. 2024 Jun 26;5 (1) :20240002.|Microbiol Spectr. 2025 Nov 5;13 (12) :e0143825.|Nanoscale. 2023 Nov 16;15 (44) :17825-17838.|Nat Immunol. 2025 Oct;26 (10) :1660-1672.|Pharmaceutics. 2023 Apr 30;15 (5) :1379.|SSRN. 2024 Nov 12.|University of Washington. 2025.|Drug Dev Ind Pharm. 2024 Aug 7:1-16.

• CAS Number:

  [144189-73-1]