Anti-Interleukin 10 (IL10) (Immunoregulation Marker) (IL10/2651R), CF594 conjugate

Anti-Interleukin 10 (IL10) (Immunoregulation Marker) (IL10/2651R), CF594 conjugate

• Description: Interleukin 10 (IL10) is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immuno-regulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immuno-regulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Cytokine Synthesis Inhibitory Factor (CSIF); GVHDS; IL-10; IL10; IL10A; Interleukin-10; T-cell Growth Inhibitory Factor (TGIF)
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: IL10
• Gene ID: 3586
• NCBI Gene ID: 193717
• UniProt: P22301
• Cellular Locus: Secreted (extracellular)
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Recombinant full-length human Interleukin 10 (IL10) protein
• Target Antigen: Interleukin 10
• Clonality: Recombinant Monoclonal
• Isotype: IgG
• Clone: IL10/2651R
• Conjugation: CF594
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Cytokines, Immunology, Inflammation
• Positive Control: SK-BR3 Cells. Adrenal, Lymph Node, Bladder, Prostate or Endometrium.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 20 kDa (Monomer); 37 kDa (Dimer)
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years