Anti-E2F-1 Antibody

CAT: 0014-3332-1Size: 100 µgDry Ice: NoHazardous: No

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CAT#:0014-3332-1Size:100 µg

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Description

Mouse Monoclonal Antibody specific to E2F-1

CAS Number

9007-83-4

Product Name Alternative

E2F-1, PBR3, Retinoblastoma-associated protein 1, RBAP-1, Retinoblastoma-binding protein 3, RBBP-3, pRB-binding protein E2F-1

Gene Name

E2F1

Gene ID

1869

NCBI Gene ID

E2F1

UniProt

Q01094

Accession Number

NP_005216

Cellular Locus

Nucleus

Host

Mouse

Reactivity

Human

Immunogen

GST fusion protein expressed in E. coli corresponding to aa 162-476 of human E2F-1.

Target Antigen

Transcription factor E2F1

Target

E2F-1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC-P, IP

Field of Research

Cancer Research

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 rPubMed:10675335, PubMed:12717439, PubMed:17050006, PubMed:17704056, PubMed:18625225, PubMed:20040599, PubMed:20176812, PubMed:28992046, PubMed:8170954}.

Additionnal Information

Immunoblotting: use at 1-5 ug/mL.

Immunoprecipitation: use at 1-5 ug/mL.

Positive controls: E2F-1 transfected Soas2 cells and recombinant fusion protein.

Storage Conditions

This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes an epitope within aa 332-390 of human E2F-1.

Formulation

PBS, pH 7.4

Buffer pH

pH 7.4

UniProtKB · Q01094

Transcription factor E2F1

E2F1_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q01094
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: E2F1
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: E2F-1
EC number: —
Processing: —
Secondary accessions: Q13143, Q92768

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationMethylationPhosphoproteinUbl conjugation

Molecular function

ActivatorDNA-binding

Biological process

ApoptosisCell cycleHost-virus interactionTranscriptionTranscription regulation

Cellular component