E2F 1 Antibody

• Background:

  Transcription activator that binds DNA cooperatively with dp proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-1 binds preferentially RB1 protein, in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent apoptosis.

• NCBI Gene ID:

  1869

• Swiss Prot:

  Q01094

• Accession Number:

  NP_005216.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  E2F-1 antibody was raised against a peptide sequence around aa.430~434 (G-D-L-T-P) derived from Human E2F-1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Apoptosis

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific peptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  70 kDa

• Specificity:

  This antibody detects endogenous levels of total E2F-1 protein.

• Applications Notes:

  Western Blot: 1:500~1:1000

• Symbol:

  E2F1

• NCBI Official Name:

  E2F transcription factor 1

• NCBI Organism:

  Homo sapiens

• Other Product Names:

  RBP3, E2F-1, RBAP1, RBBP3, RAF, RBP3

• Tested Applications:

  WB

• Protein ID:

  12669911

• Physical Properties:

  Liquid