Anti-CD103 / Integrin alpha E (T-Cell Lymphoma & Hairy Cell Leukemia Marker) (ITGAE/2474), Biotin conjugate

• Description:

  CD103 is alpha E integrin, belonging to a small beta-7 integrin subfamily. It is primarily expressed in intraepithelial lymphocytes. Together with beta 7 integrin, it is the cell surface receptor for E-cadherin, where it mediates cell adhesion. CD103 is expressed on more than 95% of intraepithelial CD8 cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  CD103; Integrin alpha E; Integrin subunit alpha E

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  ITGAE

• Gene ID:

  3682

• NCBI Gene ID:

  513867

• UniProt:

  P38570

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant human ITGAE protein fragment (exact sequence is proprietary)

• Target Antigen:

  CD103 | Integrin alpha E

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  ITGAE/2474

• Conjugation:

  Biotin

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  IHC, FFPE (verified)

• Validated Applications:

  IHC, FFPE

• Field of Research:

  Cancer, Immunology

• Positive Control:

  Tonsil, Colon or Stomach

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% BSA, 0.05% azide

• Molecular Weight:

  150 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4