CD103 Antibody / Integrin alpha E

• Description:

  This antibody recognizes a protein of 150kDa, identified as CD103, which is the alpha-E integrin subunit of the heterodimeric alpha-E-beta-7 integrin belonging to a small beta-7 integrin subfamily. CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia (HCL), the cells express CD103. This antibody is an extremely useful addition to the IHC panel for the diagnosis of hairy cell leukemia (HCL) .

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P38570

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 775-855 from the human protein was used as the immunogen for the CD103 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2c κ

• Clone:

  ITGAE/2063

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the CD103 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD103 antibody is available for research use only.

• Storage Conditions:

  Store the CD103 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Titering of the CD103 antibody may be required for optimal performance.

• CAS Number:

  9007-83-4

• Location:

  Cell membrane, cytoplasm

• Image Legend:

  IHC testing of FFPE human tonsil with CD103 antibody (clone ITGAE/2063) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.