Anti-TLE1 (Synovial Sarcoma Marker) (TLE1/2051)
CAT:
37-BNUB2051-500
Size:
500 µL
Price:
Ask
- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No
Anti-TLE1 (Synovial Sarcoma Marker) (TLE1/2051)
- Description: Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein 1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Synonyms: Enhancer of split groucho 1 (ESG1); Enhancer of split groucho-like protein 1; GRG1; TLE1; Transducin like enhancer of split 1; Transducin-like enhancer protein 1
- CAS Number: 9007-83-4
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: TLE1
- Gene ID: 7088
- NCBI Gene ID: 197320; 689805
- UniProt: Q04724
- Cellular Locus: Nucleus
- Host: Mouse
- Species Reactivity: Human
- Immunogen: Recombinant human TLE1 fragment (aa 175-338) (exact sequence is proprietary)
- Target Antigen: TLE1
- Clonality: Monoclonal
- Isotype: IgG2a κ
- Clone: TLE1/2051
- Conjugation: Purified, with BSA
- Source: Animal
- Applications: IHC, FFPE (verified) | WB (verified)
- Validated Applications: IHC, FFPE, WB
- Field of Research: Cancer, Developmental biology, Transcription factors
- Positive Control: HeLa, HepG2 or Jurkat cells. Endometrium Carcinoma or Sarcomas
- Concentration: 0.2 mg/mL
- Buffer: PBS, 0.05% BSA, 0.05% azide
- Molecular Weight: 83 kDa
- Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years