Anti-TLE1 (Synovial Sarcoma Marker) (TLE1/2946R)

Anti-TLE1 (Synovial Sarcoma Marker) (TLE1/2946R)

• Description: Key players in the Notch pathway are the TLE genes, which are human homologs of the Drosophila groucho gene. Groucho is a transcriptional repressor that plays a key role in neurogenesis, segmentation and sex determination. Transducin-like enhancer protein 1 (TLE1) is a protein that is encoded by the TLE1 gene and is involved in control of hematopoiesis, neuronal, and terminal epithelial differentiation. Positive immunohistochemical nuclear staining with anti-TLE-1 has been shown to be a useful addition to an IHC panel when differentiating synovial sarcoma from other soft tissue malignancies.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Enhancer of split groucho 1 (ESG1); Enhancer of split groucho-like protein 1; GRG1; TLE1; Transducin like enhancer of split 1; Transducin-like enhancer protein 1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: TLE1
• Gene ID: 7088
• NCBI Gene ID: 197320; 689805
• UniProt: Q04724
• Cellular Locus: Nucleus
• Host: Rabbit
• Species Reactivity: Human
• Immunogen: Recombinant human TLE1 fragment (aa 175-338) (exact sequence is proprietary)
• Target Antigen: TLE1
• Clonality: Recombinant Monoclonal
• Isotype: IgG
• Clone: TLE1/2946R
• Conjugation: Purified, with BSA
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Cancer, Developmental biology, Transcription factors
• Positive Control: HeLa, HepG2 or Jurkat cells. Endometrium Carcinoma or Sarcomas
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 83 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (formalin): 1-2 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years