Anti-CD11c (Dendritic Cell Marker) (ITGAX/2507)

Anti-CD11c (Dendritic Cell Marker) (ITGAX/2507)

• Description: This antibody recognizes a protein of 145 kDa, identified as CD11c. CD11c (ITGAX), a member of the leukointegrin family, shares the same beta subunit with other members of the leukocyte adhesion molecule family, which includes CD11a (LFA-1), CD11b (MAC-1) and CD11d (ITGAD), but has a unique alpha chain. CD11c has been shown to play a role in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T-cell proliferation by Langerhans cells. CD11c is expressed prominently on the plasma membranes of monocytes, tissue macrophages, NK cells, and most dendritic cells (DCs). A lower level of expression is also observed on neutrophils as a result of its high level of expression on most DCs. An antibody to CD11c may aid in identification of lesions with histiocytic origin. It may also been used as a marker for hairy cell leukemia in paraffin-embedded tissues.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CD11 antigen-like family member C; Complement component 3 receptor 4 subunit; Integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); Leu M5 alpha subunit; Leukocyte adhesion glycoprotein p150 95 alpha chain; Myeloid membrane antigen alpha subunit; p150/95
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: ITGAX
• Gene ID: 3687
• NCBI Gene ID: 248472
• UniProt: P20702
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant fragment of human ITGAX protein (around aa 637-827) (exact sequence is proprietary)
• Target Antigen: CD11c
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: ITGAX/2507
• Conjugation: Purified, with BSA
• Disease: Tumor
• Source: Animal
• Field of Research: Cell adhesion, Immunology
• Positive Control: THP-1 cells. Tonsil or Lymph Node
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 145 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years