Anti-GLUT-1 (Tumor Progression and Mesothelioma Marker) (GLUT1/2475)

• Description:

  This antibody recognizes a protein of 55 kDa, which is identified as GLUT-1. Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. There are many types of glucose transport carrier proteins, designated as Glut-1 to Glut-12. Glut-1 is a major glucose transporter in the mammalian blood-brain barrier. It is expressed in high density on the membranes of human erythrocytes and the brain capillaries that comprise the blood-brain barrier. Glut-1 is expressed at variable levels in many human tissues. Overexpression of Glut-1 has been linked to tumor progression or poor survival of patients with carcinomas of the colon, breast, cervical, lung, bladder and mesothelioma. Glut-1 is a sensitive and specific marker for the differentiation of malignant mesothelioma (positive) from reactive mesothelium (negative)._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  Erythrocyte/hepatoma glucose transporter; Glucose transporter type-1; GLUT1; GLUT1DS; GLUTB; GT1; GTG1; Gtg3; HepG2 glucose transporter; PED; RATGTG1; Solute carrier family 2; Solute carrier family 2, facilitated glucose transporter member 1 (SLC2A1)

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  SLC2A1

• Gene ID:

  6513

• NCBI Gene ID:

  473721

• UniProt:

  P11166

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Recombinant fragment of human GLUT1 protein (around aa 203-305) (exact sequence is proprietary)

• Target Antigen:

  GLUT-1

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  GLUT1/2475

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  Flow (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)

• Validated Applications:

  FC, IF, IHC, FFPE, WB

• Field of Research:

  Cancer, Metabolism, Transporters

• Positive Control:

  MDA-MB-231 cells or erythrocytes. Mesothelioma or breast, colon and ovarian carcinoma.

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  55 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|ELISA: 1-2 ug/mL, for coating order Ab without BSA|Immunohistology (formalin): 0.5-1 ug/mL for 30 minutes at RT|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes|Optimal dilution for a specific application should be determined by user

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4