Anti-Connexin 32 (Gap Junction Protein) (M12.13)
CAT:
37-BNUM1642-50
Size:
50 µL
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No

Anti-Connexin 32 (Gap Junction Protein) (M12.13)
- Description: This antibody recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helicaldomains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Synonyms: Charcot Marie Tooth neuropathy X linked; CMTX; CMTX1; Connexin-32; Cx32; GAP junction 28kDa liver protein; Gap junction beta-1 protein; Gap junction protein beta 1 32kD; GJB1
- UNSPSC: 41116161
- UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
- Gene Name: Gjb1
- Gene ID: 2705
- NCBI Gene ID: 333303
- UniProt: P08034
- Cellular Locus: Plasma membrane
- Host: Mouse
- Species Reactivity: Human, Mouse, Rat
- Immunogen: Rat junctional complexes
- Target Antigen: Connexin-32
- Clonality: Monoclonal
- Isotype: IgG
- Clone: M12.13
- Conjugation: Purified, BSA-free
- Source: Animal
- Applications: WB (verified)
- Validated Applications: WB
- Field of Research: Gap junctions
- Positive Control: MCF-7 Cells. Liver, Kidney, Stomach or Tonsil
- Concentration: 1 mg/mL
- Buffer: PBS, no BSA, no azide
- Molecular Weight: 27-32 kDa
- Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunohistology (frozen) 0.5-1 ug/mL|Immunofluorescence 1-2 ug/mL Flow cytometry 0.5-1 ug/million cells|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
- Shipping Conditions: Room temperature
- Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
- Shelf Life: 2 years