Anti-Connexin 32 (Gap Junction Protein) (R5.21C)

• Description:

  This antibody recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alphalpha-helicaldomains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Charcot Marie Tooth neuropathy X linked; CMTX; CMTX1; Connexin-32; Cx32; GAP junction 28kDa liver protein; Gap junction beta-1 protein; Gap junction protein beta 1 32kD; GJB1

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  Gjb1

• Gene ID:

  14618 (Mouse)

• NCBI Gene ID:

  21198 (Mouse)

• UniProt:

  P28230 (Mouse)

• Cellular Locus:

  Plasma membrane

• Host:

  Rat

• Species Reactivity:

  Mouse, Rat

• Immunogen:

  Mouse liver DOC-JR-plasma membranes

• Target Antigen:

  Connexin-32

• Clonality:

  Monoclonal

• Isotype:

  IgG2a

• Clone:

  R5.21C

• Conjugation:

  Purified, with BSA

• Source:

  Animal

• Applications:

  IF (published) | IHC, frozen (published) | IP (published) | WB (published)

• Validated Applications:

  IF, IHC, IP, WB

• Field of Research:

  Gap junctions

• Positive Control:

  MCF-7 Cells. Pancreas, Liver, Kidney, Stomach or Tonsil

• Concentration:

  0.2 mg/mL

• Buffer:

  PBS, 0.05% BSA, 0.05% azide

• Molecular Weight:

  27-32 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow cytometry: 0.5-1 ug/million cells|Immunohistology (frozen) 0.5-1 ug/mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. J Cell Biol (1986) 103 (3): 755-766. (WB) Endocrinology (1993) 133: 2371-2378. (IF) J Cell Sci (1994) 107: 955-967. (IP) Int J Cancer (1997) 73: 479-485. (IHC, frozen)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4