Anti-Connexin 32 (Gap Junction Protein) (R5.21C)

Anti-Connexin 32 (Gap Junction Protein) (R5.21C)

• Description: This antibody recognizes a protein of 27-32 kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alphalpha-helicaldomains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: Charcot Marie Tooth neuropathy X linked; CMTX; CMTX1; Connexin-32; Cx32; GAP junction 28kDa liver protein; Gap junction beta-1 protein; Gap junction protein beta 1 32kD; GJB1
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: Gjb1
• Gene ID: 14618 (Mouse)
• NCBI Gene ID: 21198 (Mouse)
• UniProt: P28230 (Mouse)
• Cellular Locus: Plasma membrane
• Host: Rat
• Species Reactivity: Mouse, Rat
• Immunogen: Mouse liver DOC-JR-plasma membranes
• Target Antigen: Connexin-32
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone: R5.21C
• Conjugation: Purified, BSA-free
• Source: Animal
• Applications: IF (published) | IHC, frozen (published) | IP (published) | WB (published)
• Validated Applications: IF, IHC, IP, WB
• Field of Research: Gap junctions
• Positive Control: MCF-7 Cells. Pancreas, Liver, Kidney, Stomach or Tonsil
• Concentration: 1 mg/mL
• Buffer: PBS, no BSA, no azide
• Molecular Weight: 27-32 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Flow cytometry: 0.5-1 ug/million cells|Immunohistology (frozen) 0.5-1 ug/mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. J Cell Biol (1986) 103 (3): 755-766. (WB) Endocrinology (1993) 133: 2371-2378. (IF) J Cell Sci (1994) 107: 955-967. (IP) Int J Cancer (1997) 73: 479-485. (IHC, frozen)
• Shipping Conditions: Room temperature
• Storage Conditions: -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years