Anti-E-Cadherin / CD324 (Intercellular Junction Marker) (4A2), Biotin conjugate

• Description: This antibody recognizes a protein of 120-80 kDa, identified as E-cadherin. Cadherins comprise a family of Ca2 -dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin ) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_
• Synonyms: Arc 1|cadherin 1 type 1 E-cadherin|Cadherin1|CAM 120/80|CD324|CDH1|CDHE|E-Cad/CTF3|E-cadherin|ECAD|Epithelial cadherin|epithelial calcium dependent adhesion protein|Liver cell adhesion molecule (LCAM)|Uvomorulin (UVO)
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CDH1
• Gene ID: 999
• NCBI Gene ID: 461086
• UniProt: P12830
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant human full-length E-Cadherin protein
• Target Antigen: CD324 | E-Cadherin
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: 4A2
• Conjugation: Biotin
• Disease: Tumor
• Source: Animal
• Applications: Flow (verified) | IF (verified) | IHC, FFPE (verified) | IP (published) | WB (verified)
• Validated Applications: FC, IF, IHC, FFPE, IP, WB
• Field of Research: Cancer, Cell adhesion
• Positive Control: LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 120-80 kDa (Mature); 135 kDa (Precursor)
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user
• References & Citations: Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Res (2005) 65: 2. (immunoprecipitation; immunofluorescence)
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years