Thermostable T7 RNA Polymerase

Thermostable T7 RNA Polymerase

• UNSPSC Description: Thermostable T7 RNA Polymerase is a thermostable version of T7 RNA Polymerase (HY-E70090). Compared with T7 RNA Polymerase, it has high temperature resistance and stable activity. T7 RNA polymerase is a polymerase expressed by Escherichia coli from the RNA polymerase gene of T7 bacteriophage. T7 RNA polymerase is highly specific and involved in in vitro transcription (IVT) of mRNA. In the presence of Mg2+, T7 RNA polymerase only uses the single-stranded or double-stranded DNA containing the T7 promoter sequence as a template, and uses NTP as a substrate to synthesize RNA complementary to the single-stranded DNA downstream of the promoter[1][2][3].
• Target Antigen: DNA/RNA Synthesis
• Type: Enzyme
• Related Pathways: Cell Cycle/DNA Damage
• Applications: Cancer-programmed cell death
• Field of Research: Others
• Assay Protocol: https://www.medchemexpress.com/thermostable-t7-rna-polymerase.html
• Smiles: [Thermostable T7 RNA Polymerase]
• References & Citations: [1]Kimoto M, et al. Genetic alphabet expansion transcription generating functional RNA molecules containing a five-letter alphabet including modified unnatural and natural base nucleotides by thermostable T7 RNA polymerase variants. Chem Commun (Camb). 2017 Nov 14;53(91):12309-12312.|[2]Dousis A, et al. An engineered T7 RNA polymerase that produces mRNA free of immunostimulatory byproducts. Nat Biotechnol. 2023 Apr;41(4):560-568.|[3]Borkotoky S, et al. The highly efficient T7 RNA polymerase: A wonder macromolecule in biological realm. Int J Biol Macromol. 2018 Oct 15;118(Pt A):49-56.
• Shipping Conditions: Room temperature
• Clinical Information: No Development Reported