Biotin-14-dCTP

• Description :

  Biotin-14-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties for Nick Translation are ensured by a 14-atom linker attached to the N4 position of cytosine. For PCR incorporation experiments e.g. with Taq polymerase Biotin-11-dCTP (#NU-809-BIOX) or Biotin-16-dCTP (#NU-809-BIO16) are recommended whose Biotin moiety is attached to C5 position of cytidine via a 11-atom or 16-atom linker, respectively.

• UNSPSC :

  12352208

• UNSPSC Description :

  Nucleic acids

• HS Code :

  29349990

• eClass Number :

  34160990

• eClass Product Group :

  Nucleinsäure (nicht klassifiziert)

• Applications :

  Incorporation into DNA/cDNA by - Nick Translation with DNAse I/ DNA Polymerase I [1] & in-house data - Primer Extension with Klenow fragment [2]

• Concentration :

  (1 mM)

• Purity :

  ≥ 95 % (HPLC)

• Form :

  Filtered solution (30 kDa) in 10 mM Tris-HCl

• pH :

  7.5 ±0.5

• Color :

  Colorless to slightly yellow

• Autoclavability :

  false

• Smiles :

  OP(=O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]1O[C@@H](n2ccc(nc2=O)NCCCCCCNC(=O)CCCCCNC(=O)CCCC[C@@H]2SC[C@@H]3NC(=O)N[C@H]23)C[C@@H]1O

• Shipping Conditions :

  Shipped on gel packs

• Storage Conditions :

  Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Storage Temperature :

  Store at -20 °C

• Notes :

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life :

  12 months after date of delivery