Biotin-16-dCTP

CAT: 0220-NU-809-BIO16-LSize: 5x 200 μLDry Ice: NoHazardous: No

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CAT#:0220-NU-809-BIO16-LSize:5x 200 μL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine.

Product Name Alternative

Biotin-16-Propargylamino-dCTP

UNSPSC

12352208

UNSPSC Description

Nucleic acids

HS Code

29349990

eClass Number

34160990

eClass Product Group

Nucleinsäure (nicht klassifiziert)

Applications

Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I [1,2] - Primer Extension with Klenow exo- [3] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [4] - Reverse Transcription with MMLV Reverse Transcriptase [5]

Concentration

(1 mM)

Purity

≥ 95 % (HPLC)

Form

Filtered solution (30 kDa) in 10 mM Tris-HCl

7.5 ±0.5

Color

Colorless to slightly yellow

Autoclavability

—

Smiles

C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NCCCC(=O)NCC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)NC(=O)N2

Shipping Conditions

Shipped on gel packs

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Storage Temperature

Store at -20 °C

Notes

Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life

12 months after date of delivery

Product Datasheet

Product MSDS

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Alternative Products

Catalog	Name
B8151-.01	Biotin-16-dCTP
JBS-NU-809-BIO16-L	Biotin-16-dCTP, L pack
N-5002	Biotin-16-Aminoallyl-2'-dCTP
JBS-NU-944-5	Biotin-16-dCTP, S pack
HY-D1668	Biotin-11-dCTP
GTR19152529	Biotin-14-dCTP
D3187-03	4-dCTP (Biotin)
D3187-03K-01	11-dCTP (Biotin)
B8153-.2	N4-Biotin-OBEA-dCTP
JBS-NU-956-BIO14-L	Biotin-14-dCTP, L pack

Biotin-16-dCTP

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