Biotin-16-dCTP

• Description :

  Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine.

• Product Name Alternative :

  Biotin-16-Propargylamino-dCTP

• Synonyms :

  Biotin-16-Propargylamino-dCTP

• UNSPSC :

  12352208

• UNSPSC Description :

  Nucleic acids

• HS Code :

  29349990

• eClass Number :

  34160990

• eClass Product Group :

  Nucleinsäure (nicht klassifiziert)

• Applications :

  Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I [1,2] - Primer Extension with Klenow exo- [3] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [4] - Reverse Transcription with MMLV Reverse Transcriptase [5]

• Concentration :

  (1 mM)

• Purity :

  ≥ 95 % (HPLC)

• Form :

  Filtered solution (30 kDa) in 10 mM Tris-HCl

• pH :

  7.5 ±0.5

• Color :

  Colorless to slightly yellow

• Autoclavability :

  false

• Smiles :

  C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NCCCC(=O)NCC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)NC(=O)N2

• Shipping Conditions :

  Shipped on gel packs

• Storage Conditions :

  Store at -20 °C Short term exposure up to 1 week cumulative to ambient temperature possible.

• Storage Temperature :

  Store at -20 °C

• Notes :

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life :

  12 months after date of delivery