Biotin-16-dCTP

• Description:

  Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine.

• Product Name Alternative:

  Biotin-16-Propargylamino-dCTP

• Synonyms:

  Biotin-16-Propargylamino-dCTP

• UNSPSC:

  12352208

• UNSPSC Description:

  Nucleic acids

• HS Code:

  29349990

• eClass Number:

  34160990

• eClass Product Group:

  Nucleinsäure (nicht klassifiziert)

• Applications:

  Incorporation into DNA/cDNA by - PCR with Taq polymerase in-house data - Nick Translation with DNAse I/ DNA Polymerase I [1,2] - Primer Extension with Klenow exo- [3] - 3’-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) [4] - Reverse Transcription with MMLV Reverse Transcriptase [5]

• Concentration:

  (1 mM)

• Purity:

  ≥ 95 % (HPLC)

• Form:

  Filtered solution (30 kDa) in 10 mM Tris-HCl

• pH:

  7.5 ±0.5

• Color:

  Colorless to slightly yellow

• Autoclavability:

  false

• Smiles:

  C1[C@H]2[C@@H] ([C@@H] (S1) CCCCC (=O) NCCCCCC (=O) NCCCC (=O) NCC#Cc1cn (c (=O) nc1N) [C@@H]1O[C@H] (COP (=O) (O) OP (=O) (O) OP (=O) (O) O) [C@@H] (O) C1) NC (=O) N2

• Shipping Conditions:

  Shipped on gel packs

• Storage Conditions:

  Store at -20 °C Short term exposure up to 1 week cumulative to ambient temperature possible.

• Storage Temperature:

  Store at -20 °C

• Notes:

  Short term exposure (up to 1 week cumulative) to ambient temperature possible.

• Shelf Life:

  12 months after date of delivery