Recombinant Mouse IL10

CAT: 0171-Z200119Size: 1 mgDry Ice: NoHazardous: No
CAT#:0171-Z200119Size:1 mg
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Description
IL-10 is produced by mouse Th2 cells following their stimulation by lectins. The main source for B cell derived IL-10 in mice are Ly1 B cells that express CD5 (Ly1) and CD11. In humans IL-10 is produced by activated CD8(+) peripheral blood T cells, by T helper CD4(+) T cell clones after both antigen-specific and polyclonal activation, by B cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. IL-10 is a homodimeric protein with subunits having a length of 160 amino acids. Human Interleukin10 shows 73% amino acid homology with mouse Interleukin-10. The murine IL-10 receptor has been cloned. This receptor is a protein of approximately 110 kDa that binds murine IL-10 specifically. This receptor is structurally related to receptors for IFN. The CD nomenclature for this receptor is CDw210. IL-10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL-2 and TNF-beta in Th1 T helper subpopulations of T cells but not of Th2 helper cells. This activity is antagonized by IL-4. The inhibitory effect on IFN- gamma production is indirect and appears to be the result of a suppression of IL-12 synthesis by accessory cells. In the human system, IL-10 is produced by, and down-regulates the function of, Th1 and Th2 cells.
CAS Number
9000-83-3
Gene Name
IL10
Gene Aliases
IL-10, Cytokine synthesis inhibitory factor, CSIF
Recombinant Mouse Interleukin-10 (IL10)
Gene ID
16153
Accession Number
P18893
Organism
Mouse
Source
E. coli
Concentration
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity
>95% as determined by SDS-PAGE
Solubility
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Function
ED50 was determined by the dose-dependent (with IL-4) proliferation of murine MC/9 cells, and was found to be < 2.0 ng/mL, corresponding to a specific activity of > 5.0 x 10^5 units/mg.
Molecular Weight
19
Shipping Conditions
Ambient Temperature
Storage Conditions
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Appearance
Lyophilized Powder
Formulation
Recombinant Interleukin-10 was lyophilized from 0.2 μm filtered PBS solution, pH 7.0.

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