Recombinant Human IL10

• Description :

  In humans, IL10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T cell clones after both antigen-specific and polyclonal activation, by B cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. Human IL10 is a homodimeric protein that shares 73% amino acid homology with mouse IL10. IL10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL2 and TNF-beta in Th1 T helper subpopulations of T cells but not of Th2 helper cells. This activity is antagonized by IL4. The inhibitory effect on IFN-gamma production is indirect and appears to be the result of a suppression of IL12 synthesis by accessory cells. In the human system, IL10 is produced by, and down-regulates the function of Th1 and Th2 cells.

• Gene Name :

  IL10

• Gene Aliases :

  IL-10, Cytokine synthesis inhibitory factor, CSIF, B-TCGF, TGIF
  Recombinant Human Interleukin-10 (IL10)

• Gene ID :

  3586

• Accession Number :

  P22301

• Organism :

  Human

• Source :

  E. coli

• Concentration :

  <1.0 EU/μg of recombinant protein as determined by the LAL method

• Purity :

  >95% as determined by SDS-PAGE

• Solubility :

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function :

  The ED50 as determined by the dose-dependent co-stimulation (with murine IL-4) of MC/9 cells was found to be ≤ 2.0 ng/mL

• Molecular Weight :

  19

• Shipping Conditions :

  Ambient Temperature

• Storage Conditions :

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance :

  Lyophilized Powder

• Formulation :

  Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.0)

• CAS Number :

  9000-83-3