Recombinant Human IL10

CAT: 0171-Z101499Size: 1 mgDry Ice: NoHazardous: No
CAT#:0171-Z101499Size:1 mg
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
IL-10 is produced by mouse Th2 cells following their stimulation by lectins. The main source for B cell derived IL-10 in mice are Ly1 B cells that express CD5 (Ly1) and CD11.In humans IL10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T cell clones after both antigen-specific and polyclonal activation, by B cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells. IL-10 is a homodimeric protein with subunits having a length of 160 amino acids. Human Interleukin 10 shows 73% amino acid homology with mouse Interleukin-10. The murine IL-10 receptor has been cloned. This receptor is a protein of approximately 110 kDa that binds murine IL-10 specifically. This receptor is structurally related to receptors for IFN. The CD nomenclature for this receptor is CDw210. IL10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL-2 and TNF-beta in Th1 T helper subpopulations of T cells but not of Th2 helper cells. This activity is antagonized by IL-4. The inhibitory effect on IFN-gamma production is indirect and appears to be the result of a suppression of IL-12 synthesis by accessory cells. In the human system, IL1-0 is produced by, and down-regulates the function of Th1 and Th2 cells.
CAS Number
9000-83-3
Gene Name
IL10
Gene Aliases
IL-10, Cytokine synthesis inhibitory factor, CSIF, B-TCGF, TGIF
Recombinant Human Interleukin-10 (IL10)
Gene ID
3586
Accession Number
P22301
Organism
Human
Source
E. coli
Concentration
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity
>95% as determined by SDS-PAGE
Solubility
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Function
The ED(50) determined by dose-dependent co-stimulation (with murine IL-4) of MC/9 cells. The ED(50) was found to be ≤ 2.0 ng/mL, corresponding to a specific activity of ≥ 4.0 x 10^5 units/mg.
Molecular Weight
19
Shipping Conditions
Ambient Temperature
Storage Conditions
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Appearance
Lyophilized Powder
Formulation
Recombinant Interleukin-10 was lyophilized from a 0.2 μm filtered PBS solution pH 7.0.

Related Products

CatalogName
Z200115Recombinant Mouse IL10
Z300029Recombinant Rat IL10
Z300025Recombinant Rat IL10
Z200119Recombinant Mouse IL10
24807051IL10 Adenovirus (Human)
24807011IL10 ORF Vector (Human) (pORF)
24807171IL10 siRNA Oligos set (Human)
ORF013338IL10 ORF Vector (Human) (pORF)
24807101IL10 AAV Vector (Human) (CMV)
24807056IL10 Adenovirus (Rat)

Alternative Products

CatalogName
MBS7614027-01Recombinant Human IL10
TP727924IL10 Human Recombinant Protein
MBS8249218-01Recombinant Human IL10 Protein
PPT-13318Human IL10 Recombinant Protein
OPCA00658-100UGIL10 Recombinant Protein (Human)
AP71941-01Recombinant Human Interleukin-10(IL10)
TP316785LIL10 (NM_000572) Human Recombinant Protein
MBS283704-01Recombinant Human Interleukin 10 (IL10)
155341-01Interleukin 10 (IL10) Recombinant, Human
MBS1573644-01Recombinant Human IL10, C-His

Popular Products