Recombinant Human MIP-3 alpha (CCL20)

• Description:

  Macrophage inflammatory protein (MIP)-3/CCL20, also known as liver and activation-regulated chemokine (LARC) or Exodus, is a member of the CC chemokine subfamily initially noted to be expressed in human liver, lung, appendix, and tonsillar crypts. MIP-3 is selectively chemotactic for CD34(+) bone marrow cell-derived immature DCs and CD45RO(+) memory T cells that express the cognate receptor CCR6. MIP-3 produced at sites of inflammation may chemoattract CCR6-expressing immature DCs to the subepithelial region of mucosal surfaces.

• Gene Name:

  CCL20

• Gene Aliases:

  Beta-chemokine exodus-1, MIP3 alpha, CC chemokine LARC, Liver and activation-regulated chemokine, MIP-3-alpha, Small-inducible cytokine A20, SCYA20
  Recombinant Human Macrophage Inflammatory Protein-3 Alpha (CCL20)

• Gene ID:

  6364

• Accession Number:

  P78556

• Organism:

  Human

• Source:

  E. coli

• Concentration:

  <1.0 EU/μg of recombinant protein as determined by the LAL method.

• Purity:

  >95% as determined by SDS-PAGE

• Solubility:

  A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

• Function:

  Determined by its ability to chemoattract human monocytes using a concentration range of 2-40 ng/mL.

• Molecular Weight:

  8

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

• Appearance:

  Lyophilized Powder

• Formulation:

  Recombinant CCL20 was lyophilized from a 0.2 μm filtered 20 mM PB,100 mM NaCl solution pH 7.5.

• CAS Number:

  9000-83-3