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Recombinant Mouse MIP-3 (CCL19)

CAT: 0171-Z200455Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0171-Z200455Size:20 µg
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Description
The chemokine MIP-3 beta is a chemoattractant for T and B lymphocytes, dendritic cells, macrophage progenitor cells, and natural killer cells. It might, therefore, play an important role in the trafficking of T cells in the thymus and migration of T and B cells to secondary lymphoid organs. Furthermore, MIP-3 beta has been recently shown to mediate rapid adhesion of naive CD4(+) T lymphocytes to activated endothelial cells supporting the role of this chemokine in regulation of lymphocyte homing. MIP-3 beta acts through CC chemokine receptor 7 (CCR7). The chemokines Lkn-1 and MIP-3 alpha are also lymphocyte attractants, although compared with MIP- 3 beta, their promigratory effect on lymphocytes is less intensive and their chemotactic activity is more promiscuous. Lkn-1 attracts monocytes, lymphocytes, and eosinophils via the chemokine receptors CCR1 and CCR3. MIP-3 alpha exhibits promigratory effects on lymphocytes, neutrophils, and immature dendritic cells via the CCR6 receptor.
CAS Number
9000-83-3
Gene Name
CCL19
Gene Aliases
C-C motif chemokine 19, MIP3, Epstein-Barr virus-induced molecule 1 ligand chemokine, EBI1 ligand chemokine, ELC, Small-inducible cytokine A19
Gene ID
24047
Accession Number
O70460
Organism
Mouse
Source
E. coli
Concentration
<1.0 EU/μg of recombinant protein as determined by the LAL method.
Purity
>95% as determined by SDS-PAGE
Solubility
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Function
Determined by its ability to chemoattract human dendritic cells using a concentration range of 10-50 ng/mL.
Molecular Weight
10
Shipping Conditions
Ambient Temperature
Storage Conditions
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Appearance
Lyophilized Powder
Formulation
Recombinant CCL19 was lyophilized from a 0.2 μm filtered 20 mM PB,100 mM NaCl solution pH 7.5.

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