Recombinant Human M-CSF

• Product Name Alternative:

  CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

• UniProt:

  P09603

• Expression Region:

  33-190

• Host:

  CHO cells

• Tag:

  C-terminal His Tag

• Applications:

  Western Blot, ELISA

• Field of Research:

  Cardiovascular, Metabolism, Immunology, stem cells

• Endotoxin:

  < 1 EU/µg as determined by LAL test.

• Purity:

  Greater than 90% as determined by SDS-PAGE.

• Activity:

  Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 2-10 ng/mL.

• Form:

  Lyophilized powder

• Reconstitution:

  Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

• Molecular Weight:

  25 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

• Formulation:

  Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.

• Organism Species:

  Human