Anti-CD162 (Selectin P Ligand) (PSGL1/1601), CF405S conjugate

Anti-CD162 (Selectin P Ligand) (PSGL1/1601), CF405S conjugate

• Description: CD162 glycoprotein functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: CD162; CLA; Cutaneous lymphocyte associated antigen; P-selectin glycoprotein lig; 1; PSGL1; Selectin P lig;; SELPLG
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: SELPLG
• Gene ID: 6404
• NCBI Gene ID: 591014
• UniProt: Q14242
• Cellular Locus: Plasma membrane
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Recombinant human full-length SELPLG (CD162) protein
• Target Antigen: CD162
• Clonality: Monoclonal
• Isotype: IgG1
• Clone: PSGL1/1601
• Conjugation: CF405S
• Source: Animal
• Applications: IHC, FFPE (verified)
• Validated Applications: IHC, FFPE
• Field of Research: Cell adhesion, Immunology
• Positive Control: Jurkat cells. Tonsil or Spleen
• Concentration: 0.1 mg/mL
• Buffer: PBS, 0.1% BSA, 0.05% azide
• Molecular Weight: 43-120 kDa
• Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin) 1-2 ug/mL|Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years