Anti-CTLA4 / CD152 (Negative Regulator of T-Cells)(L4P2F5.F10), CF740 conjugate

• Description:

  CTLA4 (CD152) is a cell surface receptor that behaves as a negative regulator of the proliferation and the effector function of T cells. It contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. The novel finding that CTLA-4 molecule is expressed and functional on human tumor cells opens up the possibility of antitumor therapeutic intervention based on targeting this molecule. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  ALPS5; CD152; Celiac disease 3 (CELIAC3); Cytotoxic T-lymphocyte-associated antigen 4 (CTLA4); GRD4; GSE; ICOS; Insulin-dependent Diabetes Mellitus 12 (IDDM12)

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CTLA4

• Gene ID:

  1493

• NCBI Gene ID:

  247824

• UniProt:

  P16410

• Cellular Locus:

  Plasma membrane|Secreted (extracellular)

• Host:

  Mouse

• Species Reactivity:

  Cow, Human, Mouse, Rat

• Immunogen:

  A synthetic peptide from CTLA4 (exact sequence is proprietary)

• Target Antigen:

  CD152 | CTLA4

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  L4P2F5.F10

• Conjugation:

  CF740

• Source:

  Animal

• Positive Control:

  Jurkat or Raji cells. Lymph Node or Tonsil.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% rBSA, 0.05% azide

• Molecular Weight:

  30-43 kDa

• Additionnal Information:

  For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years