Anti-CTLA4 / CD152 (Negative Regulator of T-Cells)(L4P2F5.F10)

Anti-CTLA4 / CD152 (Negative Regulator of T-Cells)(L4P2F5.F10)

• Description: CTLA4 (CD152) is a cell surface receptor that behaves as a negative regulator of the proliferation and the effector function of T cells. It contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases. The novel finding that CTLA-4 molecule is expressed and functional on human tumor cells opens up the possibility of antitumor therapeutic intervention based on targeting this molecule. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
• Synonyms: ALPS5; CD152; Celiac disease 3 (CELIAC3); Cytotoxic T-lymphocyte-associated antigen 4 (CTLA4); GRD4; GSE; ICOS; Insulin-dependent Diabetes Mellitus 12 (IDDM12)
• CAS Number: 9007-83-4
• UNSPSC: 41116161
• UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
• Gene Name: CTLA4
• Gene ID: 1493
• NCBI Gene ID: 247824
• UniProt: P16410
• Cellular Locus: Plasma membrane|Secreted (extracellular)
• Host: Mouse
• Species Reactivity: Cow, Human, Mouse, Rat
• Immunogen: A synthetic peptide from CTLA4 (exact sequence is proprietary)
• Target Antigen: CD152 | CTLA4
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Clone: L4P2F5.F10
• Conjugation: Purified, with BSA
• Source: Animal
• Positive Control: Jurkat or Raji cells. Lymph Node or Tonsil.
• Concentration: 0.2 mg/mL
• Buffer: PBS, 0.05% BSA, 0.05% azide
• Molecular Weight: 30-43 kDa
• Additionnal Information: For coating for ELISA, order Ab without BSA|Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Optimal dilution and staining procedure for a specific application should be determined by user|Recommended starting concentrations for titration are 1-2 ug/mL for most applications, or 1 ug/million cells/100 uL for flow cytometry
• Shipping Conditions: Room temperature
• Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
• Shelf Life: 2 years