Recombinant Human CTNNB1 Protein

• Background:

  Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome.

• Abbreviation:

  CTNNB1

• Synonyms:

  Beta-catenin; CTNNB; Catenin beta-1; OK; PRO2286; SW-cl.35

• UniProt:

  P35222

• Accession Number:

  P35222

• Expression System:

  E. coli

• Tag:

  N-His

• Sequence:

  Met131-Tyr670

• Field of Research:

  Cancer; Cardiovascular; Neuroscience; Signal Transduction

• Endotoxin:

  < 10 EU/mg of the protein as determined by the LAL method.

• Purity:

  > 85% as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  59.0 kDa

• Observed Molecular Weight:

  59 kDa

• Formulation:

  Lyophilized from sterile PBS, pH 7.4., 5% trehalose, 5% mannitol, 0.01% Tween 80.
  Normally 5%-8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human