Recombinant Human Cardiac Troponin I

• Description:

  Recombinant Human Cardiac Troponin I

• Product Name Alternative:

  TnI

• Gene Name:

  TNNI3

• UniProt:

  P19429

• Reactivity:

  Human

• Target Antigen:

  Troponin I, cardiac muscle

• Target:

  Cardiac Troponin I

• Type:

  Protein

• Applications:

  WB

• Concentration:

  Lot Specific

• Dilution:

  Dilute in PBS or medium that is identical to that used in the assay system.

• Form:

  Liquid

• Buffer:

  50mM Tris

• Function:

  Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

• Storage Conditions:

  Although stable at 4°C for 1 week, product is best stored at or below -20°C. Addition of a carrier protein (such as 0.1% HSA or BSA) is recommended for long-term storage.

• Specificity:

  Cardiac Troponin I

• Formulation:

  Sterile-filtered solution in 50mM Tris, pH 8.0 and 6M urea.

• Buffer pH:

  pH 8.0

• Target Background:

  Troponin I (TnI) troponin T (TnT) and troponin C (TnC) form the troponin complex of thin filaments of striated muscle. TnI acts as the inhibitory subunit by blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily consists of 3 genes: TnI- skeletal-fast-twitch, TnI-skeletal-slow- twitch, and TnI-cardiac. The TNNI3 gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissue. Mutations in the TNNI3 gene cause familial hypertrophic cardiomyopathy type 7 and familial restrictive cardiomyopathy.

• CAS Number:

  9000-83-3