Recombinant Human Cardiac Troponin I

Recombinant Human Cardiac Troponin I

• Description: Recombinant Human Cardiac Troponin I
• Product Name Alternative: TnI
• CAS Number: 9000-83-3
• Gene Name: TNNI3
• UniProt: P19429
• Reactivity: Human
• Target Antigen: Troponin I, cardiac muscle
• Target: Cardiac Troponin I
• Type: Protein
• Applications: WB
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Form: Liquid
• Buffer: 50mM Tris
• Function: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
• Storage Conditions: Although stable at 4°C for 1 week, product is best stored at or below -20°C. Addition of a carrier protein (such as 0.1% HSA or BSA) is recommended for long-term storage.
• Specificity: Cardiac Troponin I
• Formulation: Sterile-filtered solution in 50mM Tris, pH 8.0 and 6M urea.
• Buffer pH: pH 8.0
• Target Background: Troponin I (TnI) troponin T (TnT) and troponin C (TnC) form the troponin complex of thin filaments of striated muscle. TnI acts as the inhibitory subunit by blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily consists of 3 genes: TnI- skeletal-fast-twitch, TnI-skeletal-slow- twitch, and TnI-cardiac. The TNNI3 gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissue. Mutations in the TNNI3 gene cause familial hypertrophic cardiomyopathy type 7 and familial restrictive cardiomyopathy.