Anti-MHC II (HLA-DP) (beta chain)(BRA-FB6)

• Description:

  Recognizes a non-polymorphic determinant of DP-MHC class II. MHC class II antigens are transmembrane glycoproteins of non-covalently linkedalpha (33-35 kDa) and beta (27-30 kDa) chains. It reportedly reacts with B- & non-T, non-B cell lines but not with T- and myeloid cell lines and leukemias. Differential expression of MHC class II antigens on fetal and adult lymphocytes, malignant B cells appears to reflect the stage of cell differentiation which may be useful in the study of lymphoproliferative disorders._x000D__x000D_Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

• Synonyms:

  Ia3; Ia-3; H-2Ea; H2-Ea; AI323765; I-Ealpha; E-alpha-f; MHC-H2-Ea

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  HLA-DPB1

• Gene ID:

  3115

• NCBI Gene ID:

  347270

• UniProt:

  P04440

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human

• Immunogen:

  Non-T, non-B human acute lymphoblastic leukemia REH6 cell line

• Target Antigen:

  MHC-II

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  BRA-FB6

• Conjugation:

  Purified, BSA-free

• Source:

  Animal

• Applications:

  Flow (verified) | Flow, surface (published) | Functional studies (published) | ELISA (published) | IP (published)

• Validated Applications:

  FC, FC, ELISA, IP

• Field of Research:

  Immunology, MHC antigens

• Positive Control:

  Raji cells. Tonsil or lymph node

• Concentration:

  1 mg/mL

• Buffer:

  PBS, no BSA, no azide

• Molecular Weight:

  33-35 kDa

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Tissue Antigens (1988) 32(1):6-11. (IP) Cancer Sci (2008) 99(8): 1633-1642. (ELISPOT) World J Gastroenterol (2003) 9(5): 1077-1081. (Flow, surface) Eur J Immunol (2009) 39: 1947-1955. (functional studies)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  -35°C to -5°C ; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years

• CAS Number:

  9007-83-4