HLA-DP/DQ/DR Antibody (MHC II)

• Description:

  This mAb reacts with a common epitope of human major histocompatibility (MHC) class II antigens, HLA-DP, -DQ and -DR. Human MHC class II antigens are transmembrane glycoproteins composed of an alpha chain (36kDa) and a beta chain (27kDa) . They are expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, and thymic epithelial cells and are also present on activated T lymphocytes. Human MHC class II genes are located in the HLA-D region that encodes at least six alpha and ten beta chain genes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides and present them to T-helper cells. They, therefore, have a critical role in the initiation of the immune response. It has been shown that some auto-immune diseases are associated with certain class II alleles.

• Specifications:

  ELISA: 2-4 µg/mL for coating (order BSA/sodium azide-free format), Flow cytometry: 1-2ug/ 10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P04440, P01908, P01909, P01920, P01903

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human tonsil cells were used as the immunogen for the HLA-DP/DQ/DR antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CR3/43

• Applications:

  ELISA, FACS, IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the HLA-DP/DQ/DR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This HLA-DP/DQ/DR antibody is available for research use only.

• Storage Conditions:

  Store the HLA-DP/DQ/DR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the HLA-DP/DQ/DR antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic and cell surface

• Image Legend:

  IHC testing of FFPE human tonsil tissue with HLA-DP/DQ/DR antibody (clone CR3/43) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.