Anti-E-Cadherin / CD324 (Intercellular Junction Marker) (4A2), CF740 conjugate

• Description:

  This antibody recognizes a protein of 120-80 kDa, identified as E-cadherin. Cadherins comprise a family of Ca2 -dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin ) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma._x000D_ _x000D_ Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors._x000D_ _x000D_

• Synonyms:

  Arc 1|cadherin 1 type 1 E-cadherin|Cadherin1|CAM 120/80|CD324|CDH1|CDHE|E-Cad/CTF3|E-cadherin|ECAD|Epithelial cadherin|epithelial calcium dependent adhesion protein|Liver cell adhesion molecule (LCAM)|Uvomorulin (UVO)

• CAS Number:

  9007-83-4

• UNSPSC:

  41116161

• UNSPSC Description:

  Primary and secondary antibodies for multiple methodology immunostaining detection application

• Gene Name:

  CDH1

• Gene ID:

  999

• NCBI Gene ID:

  461086

• UniProt:

  P12830

• Cellular Locus:

  Plasma membrane

• Host:

  Mouse

• Species Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Recombinant human full-length E-Cadherin protein

• Target Antigen:

  CD324 | E-Cadherin

• Clonality:

  Monoclonal

• Isotype:

  IgG1 κ

• Clone:

  4A2

• Conjugation:

  CF740

• Disease:

  Tumor

• Source:

  Animal

• Applications:

  Flow (verified) | IF (verified) | IHC, FFPE (verified) | IP (published) | WB (verified)

• Validated Applications:

  FC, IF, IHC, FFPE, IP, WB

• Field of Research:

  Cancer, Cell adhesion

• Positive Control:

  LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.

• Concentration:

  0.1 mg/mL

• Buffer:

  PBS, 0.1% rBSA, 0.05% azide

• Molecular Weight:

  120-80 kDa (Mature); 135 kDa (Precursor)

• Additionnal Information:

  Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Western blotting 0.5-1 ug/mL|Optimal dilution for a specific application should be determined by user

• References & Citations:

  Note: References for this clone sold by other suppliers may be listed for expected applications. Cancer Res (2005) 65: 2. (immunoprecipitation; immunofluorescence)

• Shipping Conditions:

  Room temperature

• Storage Conditions:

  4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.

• Shelf Life:

  2 years