RegisterLogin

Sheep C3a (Complement Component 3a) ELISA Kit

CAT: 0855-ELK0453-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK0453-01Size:48 Tests
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep C3a in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Complement Component 3a
Reactivity
Sheep
Field of Research
Infection immunity; Immune molecule; Autoimmunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sheep C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sheep C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sheep C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sheep C3a in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
600 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
9.38-600 ng/mL
Sensitivity
3.73 ng/mL

Related Products

CatalogName
ELK10603-01Rabbit C3a (Complement Component 3a) ELISA Kit
ELK11728-01Simian C3a (Complement Component 3a) ELISA Kit
ELK10299-01Rat C3a (Complement Component 3a) ELISA Kit
ELK6030-01Dog C3a (Complement Component 3a) ELISA Kit
ELK10298-01Human C3a (Complement Component 3a) ELISA Kit
ELK10795-01Pig C3a (Complement Component 3a) ELISA Kit
ELK10300-01Mouse C3a (Complement Component 3a) ELISA Kit
ELK10299MS-01Rat C3a (Complement Component 3a) Microsample ELISA Kit
ELK10298MS-01Human C3a (Complement Component 3a) Microsample ELISA Kit
ELK10300MS-01Mouse C3a (Complement Component 3a) Microsample ELISA Kit

Alternative Products

CatalogName
MBS753322-01Sheep Complement Component 8b ELISA Kit
MBS744461-01Sheep Complement component 3b ELISA Kit
MBS012393-01Sheep Complement Component 8G ELISA Kit
MBS046267-01Sheep Complement Component 5B ELISA Kit
MBS018776-01Sheep Complement Component 4B ELISA Kit
GTR10459277Sheep Complement Component 3 ELISA kit
GTR10448028Sheep Complement Component 5 ELISA kit
GTR10456441Sheep Complement Component 9 ELISA kit
MBS735888-01Sheep Complement Component 7 ELISA Kit
MBS764484-01Sheep Complement Component 3a ELISA Kit

Popular Products