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Dog C3a (Complement Component 3a) ELISA Kit

CAT: 0855-ELK6030-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK6030-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog C3a in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Complement Component 3a
Reactivity
Dog
Field of Research
Infection immunity; Immune molecule; Autoimmunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog C3a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog C3a in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
200 ng/mL
Sample Type
Serum and plasma
Detection Range
3.13-200 ng/mL
Sensitivity
1.3 ng/mL

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