Human EIF2A (Eukaryotic Translation Initiation Factor 2 subunit alpha) ELISA Kit

Human EIF2A (Eukaryotic Translation Initiation Factor 2 subunit alpha) ELISA Kit

• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EIF2A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EIF2A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EIF2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EIF2A in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: Eukaryotic translation initiation factor 2 subunit alpha (eIF-2-alpha; eIF-2A; eIF-2alpha)
• UniProt: Q9BY44
• Field of Research: Signal transduction.
• Assay Principle: Sandwich ELISA
• Assay Performance Time: 3.5h
• Standard: 5000 pg/mL
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Detection Range: 78.13-5000 pg/mL
• Sensitivity: 32.4 pg/mL