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Human SCFR (Stem Cell Factor Receptor) ELISA Kit

CAT: 0855-ELK1182-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1182-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SCFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SCFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SCFR in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CD117; KIT; C-Kit; PBT; p145 c-Kit; C-Kit Receptor; Tyrosine-Protein Kinase; V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog; Piebald Trait
UniProt
P10721
Reactivity
Human
Field of Research
Signal transduction; Tumor immunity; Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SCFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SCFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SCFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SCFR in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.62 ng/mL

UniProtKB · P10721

Mast/stem cell growth factor receptor Kit

KIT_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P10721
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KIT
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
SCFR
EC number
2.7.10.1
Processing
Precursor
Secondary accessions
B5A956, D5LXN2, D5M931, F5H8F8, Q6IQ28, Q99662, Q9UM99
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Cellular component

Cell membraneCytoplasmMembrane

Disease

Disease variantProto-oncogene

PTM

Disulfide bondGlycoproteinPhosphoproteinUbl conjugation

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

Molecular function

KinaseReceptorTransferaseTyrosine-protein kinase