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Mouse SCFR (Stem Cell Factor Receptor) ELISA Kit

CAT: 0855-ELK2525-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2525-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SCFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SCFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SCFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SCFR in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CD117; KIT; C-Kit; PBT; p145 c-Kit; C-Kit Receptor; Tyrosine-Protein Kinase; V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog; Piebald Trait
UniProt
P05532
Reactivity
Mouse
Field of Research
Signal transduction; Tumor immunity; Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SCFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SCFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SCFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SCFR in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.062 ng/mL

UniProtKB · P05532

Mast/stem cell growth factor receptor Kit

KIT_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P05532
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Kit
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
SCFR
EC number
2.7.10.1
Processing
Precursor
Secondary accessions
Q61415, Q61416, Q61417, Q6LEE9, Q6QJB7, Q6QJB8, Q7TS86, Q8C8K9
Protein keywords

Technical term

3D-structureReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Cellular component

Cell membraneCytoplasmMembrane

Disease

Disease variantProto-oncogene

PTM

Disulfide bondGlycoproteinPhosphoproteinUbl conjugation

Domain

Immunoglobulin domainRepeatSignalTransmembraneTransmembrane helix

Molecular function

KinaseReceptorTransferaseTyrosine-protein kinase

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