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Human FE (Ferritin) ELISA Kit

CAT: 0855-ELK1214-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1214-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Ferritin
UniProt
P02792
Reactivity
Human
Field of Research
Metabolic pathway; Tumor immunity; Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FE in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.061 ng/mL

UniProtKB · P02792

Ferritin light chain

FRIL_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P02792
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
FTL
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Ferritin L subunit
EC number
—
Processing
—
Secondary accessions
B2R4B9, Q6IBT7, Q7Z2W1, Q86WI9, Q8WU07, Q96AU9, Q96CU0, Q9BTZ8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

Acetylation

Disease

CataractDisease variantNeurodegeneration

Cellular component

CytoplasmCytoplasmic vesicleLysosome

Ligand

IronMetal-binding

Biological process

Iron storage

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