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Mouse bCTx (Beta-Crosslaps) ELISA Kit

CAT: 0855-ELK1545-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1545-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse bCTx protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse bCTx in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Beta CTx; b-CTx; bCTXI; bCTX-I; B-Cr; BCL; Type I Collagen C-Telopeptide-Related Fraction
Reactivity
Mouse
Field of Research
Bone metabolism
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse bCTx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse bCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse bCTx in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
5000 pg/mL
Sample Type
Serum, plasma, urine and other biological fluids
Detection Range
78.13-5000 pg/mL
Sensitivity
27.5 pg/mL

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