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Mouse MSMb (Microseminoprotein Beta) ELISA Kit

CAT: 0855-ELK6939-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6939-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MSMb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MSMb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MSMb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MSMb in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
MSM-B; IGBF; MSP; MSPB; PN44; PRPS; PSP; PSP57; PSP94; Immunoglobulin-binding factor; Prostate secreted seminal plasma protein; plasma beta-inhibin
UniProt
O08540
Reactivity
Mouse
Field of Research
Tumor immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MSMb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MSMb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MSMb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MSMb in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.074 ng/mL

UniProtKB · O08540

Beta-microseminoprotein

MSMB_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
O08540
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Msmb
Protein existence
3: Inferred from homology
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

PTM

Disulfide bond

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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