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Human aCTx (Alpha-Crosslaps) ELISA Kit

CAT: 0855-ELK4853-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4853-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human aCTx protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human aCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aCTx in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Alpha CTx; a-CTXI; aCTX-I; A-Cr; Type I Collagen C-Telopeptide-Related Fraction
Reactivity
Human
Field of Research
Bone metabolism
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human aCTx. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human aCTx. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human aCTx in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
5000 pg/mL
Sample Type
Serum, plasma, other biological fluids
Detection Range
78.13-5000 pg/mL
Sensitivity
25.7 pg/mL

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