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Human AGRN (Agrin) ELISA Kit

CAT: 0855-ELK1870-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK1870-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AGRN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AGRN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AGRN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AGRN in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Agrin Proteoglycan
UniProt
O00468
Reactivity
Human
Field of Research
Neuro science; Developmental science; Urology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human AGRN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human AGRN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human AGRN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human AGRN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
12.9 pg/mL

UniProtKB · O00468

Agrin

AGRIN_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O00468
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
AGRN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q5SVA1, Q5SVA2, Q60FE1, Q7KYS8, Q8N4J5, Q96IC1, Q9BTD4
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

CalciumMetal-binding

Cellular component

Cell membraneExtracellular matrixMembraneSecretedSynapse

Disease

Congenital myasthenic syndromeDisease variant

Molecular function

Developmental protein

Biological process

Differentiation

PTM

Disulfide bondGlycoproteinHeparan sulfatePhosphoproteinProteoglycan

Domain

EGF-like domainLaminin EGF-like domainRepeatSignalTransmembraneTransmembrane helix

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