Human CAF (C-terminal fragement of agrin) ELISA Kit

CAT:

855-ELK9769-02

Size:

96 Tests

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Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CAF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CAF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CAF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CAF in the samples is then determined by comparing the OD of the samples to the standard curve.
CAS Number: 7732-18-5
Gene Aliases: CAF; c-terminal fragement of agrin
UniProt: Q13111
Field of Research: Neuro science; Developmental science; Urology.
Assay Principle: Sandwich ELISA
Assay Performance Time: 3.5h
Standard: 2000 pg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Detection Range: 31.25-2000 pg/mL
Sensitivity: 13.4 pg/mL

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