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Rabbit TM (Thrombomodulin) ELISA Kit

CAT: 0855-ELK2438-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2438-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit TM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit TM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit TM in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CD141; THBD; BDCA3; THRM; Fetomodulin
UniProt
Q8HZ48
Reactivity
Rabbit
Field of Research
Metabolic pathway; Hematology; Cardiovascular biology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit TM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit TM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit TM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit TM in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.056 ng/mL

UniProtKB · Q8HZ48

Thrombomodulin

Q8HZ48_RABIT · Oryctolagus cuniculus

View on UniProt ↗
Primary accession
Q8HZ48
Review status
UniProtKB unreviewed (TrEMBL)
Gene
Thbd
Protein existence
2: Evidence at transcript level
Organism
Oryctolagus cuniculus (Rabbit)
Taxonomy ID
9986
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

Biological process

Blood coagulationHemostasis

PTM

Disulfide bondGlycoproteinProteoglycan

Domain

EGF-like domainRepeatSignalTransmembraneTransmembrane helix

Ligand

Lectin

Cellular component

Membrane

Molecular function

Receptor

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