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Mouse TM (Thrombomodulin) ELISA Kit

CAT: 0855-ELK10449-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10449-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TM in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CD141; THBD; BDCA3; THRM; Fetomodulin
UniProt
P15306
Reactivity
Mouse
Field of Research
Metabolic pathway; Hematology; Cardiovascular biology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse TM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse TM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse TM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse TM in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Plasma
Detection Range
0.16-10 ng/mL
Sensitivity
0.052 ng/mL

UniProtKB · P15306

Thrombomodulin

TRBM_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P15306
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Thbd
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
TM
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Biological process

Blood coagulationHemostasis

PTM

Disulfide bondGlycoproteinProteoglycan

Domain

EGF-like domainRepeatSignalTransmembraneTransmembrane helix

Cellular component

Membrane

Molecular function

Receptor

Technical term

Reference proteome

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