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Human APOC2 (Apolipoprotein C2) ELISA Kit

CAT: 0855-ELK2740-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2740-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOC2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
APC2; Apo-C2; ProapoC-II; Apolipoprotein C-II; Proapolipoprotein C-II
UniProt
P02655
Reactivity
Human
Field of Research
Metabolic pathway; Cardiovascular biology; Hepatology; Nutrition metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOC2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
1.57-100 ng/mL
Sensitivity
0.73 ng/mL

UniProtKB · P02655

Apolipoprotein C-II

APOC2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P02655
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
APOC2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Apo-CII, ApoC-II
EC number
—
Processing
Precursor
Secondary accessions
C0JYY4, Q9BS39, Q9UDE3, Q9UNK3
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

ChylomicronHDLLDLSecretedVLDL

Disease

Disease variantHyperlipidemia

PTM

Glycoprotein

Biological process

Lipid degradationLipid metabolismLipid transportTransport

Ligand

Sialic acid

Domain

Signal

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