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Human APOL2 (Apolipoprotein L2) Microsample ELISA Kit

CAT: 0855-ELK4858MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4858MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Apo-L2; APOL-II
UniProt
Q9BQE5
Reactivity
Human
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human APOL2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human APOL2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human APOL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human APOL2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.07 ng/mL

UniProtKB · Q9BQE5

Apolipoprotein L2

APOL2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BQE5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
APOL2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B0QYK7, O95915, Q59GW9, Q5TH96, Q969T6, Q9BT28, Q9UGT1, Q9UH10
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Cellular component

Cytoplasm

Biological process

Lipid transportTransport

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